# Coscinodiscus hydrolytic enzyme activities during CDOM monoculture experiment # PI: K. Ziervogel (UNH) # version: 2018-10-17 # ========================= substrate ------------------------- a-glu ========================= sample fluor_t0 fluor_t1 fluor_t2 fluor_t3 time_t0 time_t1 time_t2 time_t3 enz_activity_t0 enz_activity_t1 enz_activity_t2 enz_activity_t3 SLOPE RSQR ------------------------- Initial (0), A1 0.51 0.43 0.95 0.56 0.00 3.00 5.50 7.60 0.00 0.00 0.01 0.00 0.000 0.159 Initial (0), A2 -0.40 -0.36 1.11 1.87 0.00 3.00 5.48 7.58 0.00 0.00 0.01 0.02 0.003 0.870 Initial (0), A3 0.79 0.55 0.32 -0.16 0.00 2.97 5.48 7.57 0.01 0.00 0.00 0.00 -0.001 0.934 Initial (0), B1 1.47 -0.83 -0.04 1.98 0.00 2.93 5.43 7.50 0.01 -0.01 0.00 0.02 0.001 0.025 Initial (0), B2 -0.08 0.63 -0.08 1.47 0.00 2.98 5.45 7.50 0.00 0.00 0.00 0.01 0.001 0.439 Initial (0), B3 -0.24 0.48 0.20 1.95 0.00 3.03 5.45 7.50 0.00 0.00 0.00 0.02 0.002 0.686 Initial (0), C1 1.15 0.47 -0.76 1.71 0.00 3.02 5.45 7.52 0.01 0.00 -0.01 0.01 0.000 0.000 Initial (0), C2 1.74 0.95 1.11 0.99 0.00 3.02 5.43 7.50 0.02 0.01 0.01 0.01 -0.001 0.604 Initial (0), C3 0.00 0.16 1.71 0.79 0.00 3.02 5.43 7.52 0.00 0.00 0.01 0.01 0.002 0.460 Exponential (21), A1 94.03 139.54 195.76 233.79 0.00 2.30 5.22 7.15 0.05 0.08 0.12 0.14 0.012 1.000 Exponential (21), A2 94.30 147.25 214.55 264.05 0.00 2.33 5.22 7.15 0.05 0.09 0.13 0.16 0.015 0.999 Exponential (21), A3 99.15 146.30 206.28 248.11 0.00 2.32 5.20 7.12 0.06 0.09 0.12 0.15 0.013 1.000 Exponential (21), B1 98.72 185.91 303.61 371.72 0.00 2.30 5.20 7.12 0.06 0.11 0.19 0.23 0.025 0.999 Exponential (21), B2 102.44 166.39 262.55 355.45 0.00 2.27 5.17 7.08 0.06 0.10 0.16 0.22 0.022 0.988 Exponential (21), B3 96.87 161.66 258.41 337.51 0.00 2.27 5.17 7.08 0.05 0.10 0.16 0.21 0.021 0.995 Exponential (21), C1 99.00 144.85 192.25 223.09 0.00 2.25 5.18 7.07 0.06 0.09 0.12 0.14 0.011 0.997 Exponential (21), C2 99.48 154.07 221.58 270.24 0.00 2.23 5.15 7.05 0.06 0.09 0.13 0.17 0.015 1.000 Exponential (21), C3 100.66 158.74 219.75 262.37 0.00 2.23 5.15 7.03 0.06 0.09 0.13 0.16 0.014 0.998 Control (0), A 24.98 5.92 85.73 87.97 0.00 3.38 5.83 7.93 0.01 0.00 0.05 0.05 0.006 0.630 Control (0), B 20.24 13.05 85.94 82.62 0.00 3.35 5.82 7.93 0.01 0.00 0.05 0.05 0.006 0.703 Control (0), C 14.85 18.98 83.65 82.05 0.00 3.33 5.82 7.92 0.00 0.01 0.05 0.05 0.006 0.799 ========================= substrate ------------------------- b-glu ========================= sample fluor_t0 fluor_t1 fluor_t2 fluor_t3 time_t0 time_t1 time_t2 time_t3 enz_activity_t0 enz_activity_t1 enz_activity_t2 enz_activity_t3 SLOPE RSQR ------------------------- Initial (0), A1 0.00 86.20 109.81 138.21 0.00 0.05 0.06 0.08 0.00 2.85 5.25 7.38 0.01 0.99 Initial (0), A2 0.00 89.93 118.00 161.83 0.00 0.05 0.07 0.10 0.00 2.80 5.20 7.32 0.01 0.97 Initial (0), A3 0.00 86.11 116.29 153.06 0.00 0.05 0.07 0.09 0.00 2.80 5.20 7.33 0.01 0.99 Initial (0), B1 0.00 92.46 121.12 161.64 0.00 0.05 0.07 0.10 0.00 2.80 5.20 7.32 0.01 0.98 Initial (0), B2 0.00 85.57 112.99 151.24 0.00 0.05 0.07 0.09 0.00 2.80 5.20 7.32 0.01 0.98 Initial (0), B3 0.00 110.82 133.76 186.94 0.00 0.06 0.08 0.11 0.00 2.78 5.22 7.32 0.01 0.93 Initial (0), C1 0.00 85.86 112.89 152.03 0.00 0.05 0.07 0.09 0.00 2.75 5.18 7.33 0.01 0.98 Initial (0), C2 0.00 87.52 117.50 166.81 0.00 0.05 0.07 0.10 0.00 2.72 5.15 7.30 0.01 0.97 Initial (0), C3 0.00 86.90 112.45 148.96 0.00 0.05 0.06 0.09 0.00 2.72 5.13 7.30 0.01 0.98 Exponential (21), A1 87.82 135.98 200.68 254.51 0.05 0.08 0.12 0.16 0.00 1.95 4.85 6.75 0.02 1.00 Exponential (21), A2 85.33 132.58 210.92 272.90 0.05 0.08 0.13 0.17 0.00 1.93 4.85 6.73 0.02 1.00 Exponential (21), A3 83.31 135.54 221.31 284.27 0.05 0.08 0.13 0.17 0.00 1.85 4.85 6.73 0.02 1.00 Exponential (21), B1 86.97 166.15 282.24 390.75 0.05 0.10 0.17 0.24 0.00 1.90 4.85 6.73 0.03 0.99 Exponential (21), B2 88.67 159.64 270.02 365.25 0.05 0.09 0.17 0.23 0.00 1.88 4.83 6.73 0.03 1.00 Exponential (21), B3 87.79 175.18 316.45 424.08 0.05 0.10 0.19 0.26 0.00 1.88 4.82 6.73 0.03 1.00 Exponential (21), C1 87.65 155.57 245.43 308.12 0.05 0.09 0.15 0.19 0.00 1.88 4.82 6.72 0.02 1.00 Exponential (21), C2 88.08 149.01 236.97 299.65 0.05 0.09 0.14 0.18 0.00 1.87 4.82 6.72 0.02 1.00 Exponential (21), C3 87.84 144.54 226.71 282.61 0.05 0.09 0.14 0.17 0.00 1.87 4.82 6.72 0.02 1.00 Control (0), A 67.84 67.05 80.99 70.74 0.04 0.04 0.04 0.04 0.00 2.90 5.28 7.43 0.00 0.22 Control (0), B nd 67.54 69.08 71.56 -0.01 0.04 0.04 0.04 0.00 2.85 5.23 7.40 0.01 0.70 Control (0), C nd 67.02 69.05 68.83 -0.01 0.04 0.04 0.04 0.00 2.87 5.25 7.40 0.01 0.68 ========================= substrate ------------------------- leu ========================= sample fluor_t0 fluor_t1 fluor_t2 fluor_t3 time_t0 time_t1 time_t2 time_t3 enz_activity_t0 enz_activity_t1 enz_activity_t2 enz_activity_t3 SLOPE RSQR ------------------------- Initial (0), A1 30.17 31.79 34.86 40.65 0.00 2.35 4.73 7.17 373.83 393.48 430.58 500.50 17.50 0.94 Initial (0), A2 28.54 32.58 35.98 37.49 0.00 2.32 4.72 7.15 354.16 403.02 444.16 462.34 15.30 0.96 Initial (0), A3 31.60 33.47 36.75 39.63 0.00 2.33 4.72 7.15 391.20 413.72 453.39 488.19 13.88 0.99 Initial (0), B1 14.51 20.15 23.63 26.44 0.00 2.30 4.68 7.25 184.66 252.85 294.89 328.82 19.57 0.97 Initial (0), B2 31.41 34.32 36.74 41.04 0.00 2.35 4.68 7.25 388.89 424.10 453.28 505.21 15.74 0.99 Initial (0), B3 32.30 34.04 37.31 38.91 0.00 2.30 4.67 7.22 399.63 420.70 460.15 479.46 11.60 0.98 Initial (0), C1 29.53 35.50 36.69 39.69 0.00 2.28 4.60 7.17 366.12 438.28 452.67 488.89 16.00 0.91 Initial (0), C2 30.44 32.60 36.66 40.31 0.00 2.27 4.60 7.23 377.18 403.31 452.28 496.38 16.94 0.99 Initial (0), C3 29.69 32.83 35.55 39.55 0.00 2.27 4.58 7.20 368.08 406.08 438.86 487.25 16.34 1.00 Exponential (21), A1 645.69 825.34 1061.59 1205.97 0.00 1.65 4.55 6.48 790.09 1008.23 1295.08 1470.39 103.72 0.99 Exponential (21), A2 654.83 824.60 1072.04 nd 0.00 1.65 4.57 6.52 801.19 1007.33 1307.77 nd 110.02 1.00 Exponential (21), A3 638.29 796.91 1013.63 1130.81 0.00 1.62 4.53 6.50 781.11 973.71 1236.85 1379.13 91.42 0.99 Exponential (21), B1 633.85 1099.15 1746.21 2074.20 0.00 1.60 4.53 6.47 775.72 1340.68 2126.34 2524.58 269.15 0.99 Exponential (21), B2 632.97 914.43 1315.63 1523.74 0.00 1.60 4.52 6.48 774.65 1116.40 1603.54 1856.22 166.31 0.99 Exponential (21), B3 650.49 955.35 1346.62 1561.86 0.00 1.58 4.52 6.47 795.92 1166.08 1641.16 1902.50 168.94 0.99 Exponential (21), C1 630.32 914.57 1309.99 1492.88 0.00 1.57 4.53 6.47 771.43 1116.57 1596.68 1818.74 161.28 0.99 Exponential (21), C2 644.47 901.84 1237.50 1417.33 0.00 1.57 4.53 6.48 788.61 1101.12 1508.66 1727.01 142.90 0.99 Exponential (21), C3 648.83 881.84 1234.59 1379.26 0.00 1.57 4.48 6.48 793.90 1076.83 1505.13 1680.78 137.77 0.98 Control (21), A 28.99 27.81 29.92 29.62 0.00 2.60 5.03 7.40 359.62 345.32 370.88 367.27 1.94 0.30 Control (21), B 30.24 30.43 31.71 33.55 0.00 2.50 4.90 7.30 374.68 376.99 392.46 414.73 5.56 0.89 Control (21), C 29.54 29.29 31.87 30.73 0.00 2.48 4.88 7.28 366.23 363.31 394.44 380.61 3.06 0.45